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Erik Sontheimer
Vincent Gerbasi
Karen Kim-Guisbert
Sara Layman
Luciano Marraffini
Jon Preall
Noelle Wakefield

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Principal Investigator

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EDUCATION

RESEARCH

PUBLICATIONS

Preall, J. B., He, Z., Gorra, J., and Sontheimer, E. J. (2006). Short Interfering RNA Strand Selection is Independent of dsRNA Processing Polarity During RNAi in Drosophila. Curr. Biol. 16, 530-535.

Bellare, P., Kutach, A. K., Rines, A., Guthrie, C., and Sontheimer, E. J. (2006). Ubiquitin Binding by a Variant Jab1/MPN Domain in the Essential Pre-mRNA Splicing Factor Prp8p. RNA 12, 292-302.

Preall, J. B., and Sontheimer, E. J. (2005). RNAi: RISC Gets Loaded. Cell 123, 543-545.

Pham, J. W., and Sontheimer, E. J. (2005). Molecular Requirements for RISC Assembly in the Drosophila RNAi Pathway. J. Biol. Chem. 280, 39278-39283.

Pellino, J. L., Jaskiewicz, L., Filipowicz, W., and Sontheimer, E. J. (2005). A Dicer-Containing Complex Interacts with Short Interfering RNAs in Human Cell Extracts. RNA 11, 1719-1724.

Sontheimer, E. J. and Carthew, R. W. (2005). Silencing From Within: Endogenous siRNAs and miRNAs. Cell 122, 9-12.

Pham, J. W. and Sontheimer, E. J. (2005). Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis. In Methods in Molecular Biology, Volume 309 (G. Carmichael, Ed.). Humana Press, Totawa, New Jersey.

Sontheimer, E. J. (2005). RNA Silencing Complex Assembly and Function. Nature Reviews Molecular and Cellular Biology 6, 127-138.

Pham, J. W. and Sontheimer, E. J. (2004). The Making of an siRNA. Molecular Cell 15, 163-164.

Sontheimer, E. J. and Carthew, R. W. (2004). Argonaute Journeys Into the Heart of RISC. Science 305, 1409-1410.

He, Z., and Sontheimer, E. J. (2004). “siRNAs and miRNAs”: A Meeting Review on RNA Silencing. RNA 10, 1165-1173.

Pham, J. W., Radhakrishnan, I., and Sontheimer, E. J. (2004). Thermodynamic and Structural Characterization of 2’-Nitrogen-modified RNA Duplexes. Nucleic Acids Research 32, 3446-3455.

Pham, J. W., Pellino, J. L., Lee, Y. S., Carthew, R. W., and Sontheimer, E. J. (2004). A Dicer-2-Dependent 80S Complex Cleaves Targeted mRNAs during RNAi in Drosophila. Cell 117, 83-94.

Lee, Y. S., Nakahara, K., Pham, J. W., Kim, K., He, Z., Sontheimer, E. J., and Carthew, R. W. (2004). Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways. Cell 117, 69-81.

Sontheimer, E. J. (2003). R2D2 Leads the Silencing Trigger to mRNA's Death Star. Cell 115, 132-133.

Sontheimer, E. J. (2001). The Spliceosome Shows its Metal. Nature Structural Biology 8, 11-13.

Gordon, P. M., Sontheimer, E. J., and Piccirilli, J. A. (2000). Kinetic Characterization of the Second Step of Group II Intron Splicing: Role of Metal Ions and the Cleavage Site 2-OH in Catalysis. Biochemistry 39, 12939-12952.

Gordon, P. M., Sontheimer, E. J., and Piccirilli, J. A. (2000). Metal Ion Catalysis During the Exon Ligation Step of Nuclear Pre-mRNA Splicing: Extending the Parallels Between the Spliceosome and Group II Introns. RNA 6, 199-205.

Warnecke, J. M., Sontheimer, E. J., Piccirilli, J. A., and Hartmann, R. K. (2000). Active Site Constraints in the Hydrolysis Reaction Catalyzed by Bacterial RNase P: Analysis of Precursor tRNAs with a Single 3-S-Phosphorothiolate Internucleotide Linkage. Nucleic Acids Research 28, 720-727.

Sontheimer, E. J., Gordon, P. M., and Piccirilli, J. A. (1999). Metal Ion Catalysis During Group II Intron Self-Splicing: Parallels with the Spliceosome. Genes and Development 13: 1729-1741.

Sontheimer, E. J. (1999). 3-Sulfur Substitution in the Analysis of Pre-mRNA Splicing. Methods 18: 29-37.

Sontheimer, E. J., Sun, S., and Piccirilli, J. A. (1997). Metal Ion Catalysis During Splicing of Premessenger RNA. Nature 388, 801-805.

Sontheimer, E. J. (1994). Site-Specific RNA Crosslinking with 4-thiouridine. Molecular Biology Reports 20, 35-44.

Sontheimer, E. J., and Steitz, J. A. (1993). The U5 and U6 Small Nuclear RNAs as Active Site Components of the Spliceosome. Science 262, 1989-1996.

Cortes, J. J., Sontheimer, E. J., Seiwert, S. D., and Steitz, J. A. (1993). Mutations in the Conserved Loop of Human U5 snRNA Generate Use of Novel Cryptic 5' Splice Sites in vivo. EMBO Journal 12, 5181-5189.

Wyatt, J. R., Sontheimer, E. J., and Steitz, J. A. (1992). Site-Specific Crosslinking of Mammalian U5 snRNP to the 5' Splice Site Prior to the First Step of Premessenger RNA Splicing. Genes and Development 6, 2542-2553.

Gelpi, C., Sontheimer, E. J., and Rodriguez-Sanchez, J. L. (1992). Autoantibodies Against a Serine tRNA-Protein Complex Implicated in Cotranslational Selenocysteine Insertion. Proceedings of the National Academy of Sciences U.S.A. 89, 9739-9743.

Sontheimer, E. J., and Steitz, J. A. (1992). Three Novel Functional Variants of Human U5 Small Nuclear RNA. Molecular and Cell Biology 12, 734-746.

HONORS / PROFESSIONAL MEMBERSHIPS

Northwestern University, Weinberg College of Arts and Sciences Distinguished Teaching Award
Research Scholar Award, American Cancer Society
Semifinalist, W. M. Keck Foundation Distinguished Young Scholars in Medical Research
CAREER Award, National Science Foundation
Basil O'Connor Starter Scholar Research Award, March of Dimes
Burroughs Wellcome Fund New Investigator Award in the Basic Pharmacological Sciences
Fellow, The Jane Coffin Childs Memorial Fund for Medical Research

ACADEMIC ACTIVITIES

BMBCB